scf (25 ng Search Results


recombinant murine scf  (ImmunoTools)
90
ImmunoTools recombinant murine scf
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stem cell factor scf  (Biovitrum)
86
Biovitrum stem cell factor scf
Stem Cell Factor Scf, supplied by Biovitrum, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stem cell factor scf  (ImmunoTools)
90
ImmunoTools stem cell factor scf
Stem Cell Factor Scf, supplied by ImmunoTools, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stemspan  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc stemspan
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mouse recombinant stem cell factor scf  (R&D Systems)
95
R&D Systems mouse recombinant stem cell factor scf
Mouse Recombinant Stem Cell Factor Scf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein 90 alpha sience icoslg inducible t cell sinobiological  (Sino Biological)
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Protein 90 Alpha Sience Icoslg Inducible T Cell Sinobiological, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human stem cell factor  (R&D Systems)
96
R&D Systems recombinant human stem cell factor
Recombinant Human Stem Cell Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human scf r cd117 mouse monoclonal antibody  (R&D Systems)
90
R&D Systems anti human scf r cd117 mouse monoclonal antibody
Figure 3 Stem cell factor may rescue K562 cells from BCR–ABL inhibition. (a) Apoptosis induced by 2 mM imatinib or 20 nM nilotinib over 48 h in K562 cells cultured either in the presence of fetal calf serum (FCS) alone (10% v/v) or in the presence of FCS and cytokines (FCS þ CK). Apoptosis was analyzed by flow cytometry using DiOC6(3) (3,3-dihexyloxacarbocyanine iodide)3 as a Dcm probe. (b) Effect of blocking <t>anti-CD117</t> antibody on the apoptosis induced over 24 h by 20 nM nilotinib in K562 cells cultured in the presence of cytokines (FCS þ CK). (c) Effect of increasing concentrations of nilotinib (black diamonds) alone or in the presence of cytokines (white squares) and cytokines þ anti-CD117 antibody (white triangles) on viable K562 cells. The thiazolyl blue tetrazolium bromide (MTT) method was used after 72 h culturing. The results were expressed as percentage of control optical density±s.d. (d) Effect of high concentration of nilotinib on the apoptosis of K562 cells cultured in either the presence or in the absence of cytokines. (e) Effect of BCR–ABL knockdown on apoptosis and proliferation of K562 cells cultured for 3 days either in the presence of FCS alone or in the presence of 200 ng/ml of SCF (FCS þ SCF). After the depletion of BCR–ABL (see inset) with shRNA, the cells were counted before and after 3 days of culturing and the proliferative index was calculated (gray bars). Spontaneous apoptosis was determined as described in (a) (white bars). All figures represent mean±s.d. of three experiments.
Anti Human Scf R Cd117 Mouse Monoclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scf  (R&D Systems)
95
R&D Systems scf
Figure 3 Stem cell factor may rescue K562 cells from BCR–ABL inhibition. (a) Apoptosis induced by 2 mM imatinib or 20 nM nilotinib over 48 h in K562 cells cultured either in the presence of fetal calf serum (FCS) alone (10% v/v) or in the presence of FCS and cytokines (FCS þ CK). Apoptosis was analyzed by flow cytometry using DiOC6(3) (3,3-dihexyloxacarbocyanine iodide)3 as a Dcm probe. (b) Effect of blocking <t>anti-CD117</t> antibody on the apoptosis induced over 24 h by 20 nM nilotinib in K562 cells cultured in the presence of cytokines (FCS þ CK). (c) Effect of increasing concentrations of nilotinib (black diamonds) alone or in the presence of cytokines (white squares) and cytokines þ anti-CD117 antibody (white triangles) on viable K562 cells. The thiazolyl blue tetrazolium bromide (MTT) method was used after 72 h culturing. The results were expressed as percentage of control optical density±s.d. (d) Effect of high concentration of nilotinib on the apoptosis of K562 cells cultured in either the presence or in the absence of cytokines. (e) Effect of BCR–ABL knockdown on apoptosis and proliferation of K562 cells cultured for 3 days either in the presence of FCS alone or in the presence of 200 ng/ml of SCF (FCS þ SCF). After the depletion of BCR–ABL (see inset) with shRNA, the cells were counted before and after 3 days of culturing and the proliferative index was calculated (gray bars). Spontaneous apoptosis was determined as described in (a) (white bars). All figures represent mean±s.d. of three experiments.
Scf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scf+(25+ng/Recombinant+Mouse+SCF+Protein/pmc07763653-16-4-6
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mouse scf  (Miltenyi Biotec)
93
Miltenyi Biotec mouse scf
Figure 3 Stem cell factor may rescue K562 cells from BCR–ABL inhibition. (a) Apoptosis induced by 2 mM imatinib or 20 nM nilotinib over 48 h in K562 cells cultured either in the presence of fetal calf serum (FCS) alone (10% v/v) or in the presence of FCS and cytokines (FCS þ CK). Apoptosis was analyzed by flow cytometry using DiOC6(3) (3,3-dihexyloxacarbocyanine iodide)3 as a Dcm probe. (b) Effect of blocking <t>anti-CD117</t> antibody on the apoptosis induced over 24 h by 20 nM nilotinib in K562 cells cultured in the presence of cytokines (FCS þ CK). (c) Effect of increasing concentrations of nilotinib (black diamonds) alone or in the presence of cytokines (white squares) and cytokines þ anti-CD117 antibody (white triangles) on viable K562 cells. The thiazolyl blue tetrazolium bromide (MTT) method was used after 72 h culturing. The results were expressed as percentage of control optical density±s.d. (d) Effect of high concentration of nilotinib on the apoptosis of K562 cells cultured in either the presence or in the absence of cytokines. (e) Effect of BCR–ABL knockdown on apoptosis and proliferation of K562 cells cultured for 3 days either in the presence of FCS alone or in the presence of 200 ng/ml of SCF (FCS þ SCF). After the depletion of BCR–ABL (see inset) with shRNA, the cells were counted before and after 3 days of culturing and the proliferative index was calculated (gray bars). Spontaneous apoptosis was determined as described in (a) (white bars). All figures represent mean±s.d. of three experiments.
Mouse Scf, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sfemii  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc sfemii
Figure 3 Stem cell factor may rescue K562 cells from BCR–ABL inhibition. (a) Apoptosis induced by 2 mM imatinib or 20 nM nilotinib over 48 h in K562 cells cultured either in the presence of fetal calf serum (FCS) alone (10% v/v) or in the presence of FCS and cytokines (FCS þ CK). Apoptosis was analyzed by flow cytometry using DiOC6(3) (3,3-dihexyloxacarbocyanine iodide)3 as a Dcm probe. (b) Effect of blocking <t>anti-CD117</t> antibody on the apoptosis induced over 24 h by 20 nM nilotinib in K562 cells cultured in the presence of cytokines (FCS þ CK). (c) Effect of increasing concentrations of nilotinib (black diamonds) alone or in the presence of cytokines (white squares) and cytokines þ anti-CD117 antibody (white triangles) on viable K562 cells. The thiazolyl blue tetrazolium bromide (MTT) method was used after 72 h culturing. The results were expressed as percentage of control optical density±s.d. (d) Effect of high concentration of nilotinib on the apoptosis of K562 cells cultured in either the presence or in the absence of cytokines. (e) Effect of BCR–ABL knockdown on apoptosis and proliferation of K562 cells cultured for 3 days either in the presence of FCS alone or in the presence of 200 ng/ml of SCF (FCS þ SCF). After the depletion of BCR–ABL (see inset) with shRNA, the cells were counted before and after 3 days of culturing and the proliferative index was calculated (gray bars). Spontaneous apoptosis was determined as described in (a) (white bars). All figures represent mean±s.d. of three experiments.
Sfemii, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scf+(25+ng/flt+3+ligand/pmc08999576-51-23-28
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Image Search Results


Figure 3 Stem cell factor may rescue K562 cells from BCR–ABL inhibition. (a) Apoptosis induced by 2 mM imatinib or 20 nM nilotinib over 48 h in K562 cells cultured either in the presence of fetal calf serum (FCS) alone (10% v/v) or in the presence of FCS and cytokines (FCS þ CK). Apoptosis was analyzed by flow cytometry using DiOC6(3) (3,3-dihexyloxacarbocyanine iodide)3 as a Dcm probe. (b) Effect of blocking anti-CD117 antibody on the apoptosis induced over 24 h by 20 nM nilotinib in K562 cells cultured in the presence of cytokines (FCS þ CK). (c) Effect of increasing concentrations of nilotinib (black diamonds) alone or in the presence of cytokines (white squares) and cytokines þ anti-CD117 antibody (white triangles) on viable K562 cells. The thiazolyl blue tetrazolium bromide (MTT) method was used after 72 h culturing. The results were expressed as percentage of control optical density±s.d. (d) Effect of high concentration of nilotinib on the apoptosis of K562 cells cultured in either the presence or in the absence of cytokines. (e) Effect of BCR–ABL knockdown on apoptosis and proliferation of K562 cells cultured for 3 days either in the presence of FCS alone or in the presence of 200 ng/ml of SCF (FCS þ SCF). After the depletion of BCR–ABL (see inset) with shRNA, the cells were counted before and after 3 days of culturing and the proliferative index was calculated (gray bars). Spontaneous apoptosis was determined as described in (a) (white bars). All figures represent mean±s.d. of three experiments.

Journal: Leukemia

Article Title: The stem cell factor-c-KIT pathway must be inhibited to enable apoptosis induced by BCR-ABL inhibitors in chronic myelogenous leukemia cells.

doi: 10.1038/leu.2008.364

Figure Lengend Snippet: Figure 3 Stem cell factor may rescue K562 cells from BCR–ABL inhibition. (a) Apoptosis induced by 2 mM imatinib or 20 nM nilotinib over 48 h in K562 cells cultured either in the presence of fetal calf serum (FCS) alone (10% v/v) or in the presence of FCS and cytokines (FCS þ CK). Apoptosis was analyzed by flow cytometry using DiOC6(3) (3,3-dihexyloxacarbocyanine iodide)3 as a Dcm probe. (b) Effect of blocking anti-CD117 antibody on the apoptosis induced over 24 h by 20 nM nilotinib in K562 cells cultured in the presence of cytokines (FCS þ CK). (c) Effect of increasing concentrations of nilotinib (black diamonds) alone or in the presence of cytokines (white squares) and cytokines þ anti-CD117 antibody (white triangles) on viable K562 cells. The thiazolyl blue tetrazolium bromide (MTT) method was used after 72 h culturing. The results were expressed as percentage of control optical density±s.d. (d) Effect of high concentration of nilotinib on the apoptosis of K562 cells cultured in either the presence or in the absence of cytokines. (e) Effect of BCR–ABL knockdown on apoptosis and proliferation of K562 cells cultured for 3 days either in the presence of FCS alone or in the presence of 200 ng/ml of SCF (FCS þ SCF). After the depletion of BCR–ABL (see inset) with shRNA, the cells were counted before and after 3 days of culturing and the proliferative index was calculated (gray bars). Spontaneous apoptosis was determined as described in (a) (white bars). All figures represent mean±s.d. of three experiments.

Article Snippet: When indicated, the cells were also incubated with 200 ng/ml human stem cell factor (R&D Systems Europe, Lille, France) or with 25 ng/ml anti-human SCF R (CD117) mouse monoclonal antibody (clone-47233, human stem cell factor, R&D Systems Europe, Lille, France).

Techniques: Inhibition, Cell Culture, Cytometry, Blocking Assay, Control, Concentration Assay, Knockdown, shRNA